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Membrane fouling is a major issue in many membrane applications. There are numerous methods used in attempt to mitigate membrane fouling, with one method being membrane surface patterning. However, it is still unclear how the ratio of foulant size to pattern size affects membrane fouling. In this study, we investigated constant foulant size while varying the pattern size on the membrane surface to be smaller than (300-nm), equal to (10-μm), and larger than (50-μm) the foulant (10-μm) on polyamide nanofiltration membranes. These membranes were compared to a commercial nanofiltration membrane and a control flat synthesized membrane. The membranes were tested with water, 2000 ppm Na2SO4, and three cycles of a n-dodecane (as oil) brine solution in a dead-end cell to assess the fouling resistance and flux recovery ability of each polyamide membrane type. From the fouling experiments, it was determined that none of the pattern sizes significantly affect the flux recovery ratio, but smaller than and larger than patterns decreased the fouling rate on the polyamide membranes by a small margin.